Themes of Patachitra explore the relationship between sacred and profane, and the boundaries between religions. Themes of stories present a local interpretation of the world seen through a mythological framework that is projected onto current events. Patachitra is used as a communication tool and to create content for graphic novels and animation as well as to spread awareness on subjects like environment, child marriage, transgender etc.

Themes of stories can be mythical and religious: Manasa Mangal, Satya Pir as well as social issues like environment, dowry, women empowerment. There is increased willingness among the artists to adapt themes based on current events.